Mushroom cultivation on agar plates

Growing mushrooms at home is a great hobby for both food, fun and profit. You will need some special equipment for the agar method need. Mushroom cultivation can be done without this step, but it is for the enthusiasts, clone the mushrooms and want to isolate major substrain of fruit bodies m
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ushrooms to save the personal culture libraries.





Agar culture:


Agar is a polysaccharide obtained from marine red algae. Agar is unique because it isAbility to stay up to cool below 36 degrees C. The advantage here is that liquid nutrients and other growth media may be mixed together before the agar solidifies. The agar will remain solid at room temperature. Fungal spores, a liquid culture, a wedge from a colonized agar petri dish or living tissue from the fungus itself: Once the solution is mixed, it can be poured into individual petri dishes for use in vaccination with one of the following. With living tissue is oneMethod for cloning a desired type and the acquisition of its unique properties.


There are 5 different types of media and its name refers to their functionality.





•   General Purpose Medium: This is growing for a broad spectrum of microorganisms. Nutrient Agar is a general purpose media.


•   Enriched medium: This medium is enriched with some kind of special growth factors, such as blood, serum, hemoglobin, etc.


•   Selective medium:This contains a chemical that inhibits the growth of certain organisms while promoting the growth of others.


•   differential medium: This can grow different types of microorganisms, but can distinguish among them by various appearances on media, color of the colonies or grow the color of the medium in which the organisms.


•   fermentation medium: This determines whether a microorganism can ferment a particular carbohydrate. Fermentation is an anaerobicProcess that often produces acids. If the carbohydrate is fermented, acid is produced, and this can be detected by a pH indicator dye.





For our purposes, we are growing mushrooms with a selective medium. We are also various nutrients, minerals, etc., to promote the growth of mycelium. We can even antibiotics inhibit the growth of some bacteria.


There are many agar recipes that can be used, but two of the most common are Potato DextroseAgar (PDA) and malt extract agar (MEA).


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Malt extract agar (MEA)


10 grams light malt extract


9 grams agar-agar


500 ml drinking water or distilled water





Potato dextrose agar (PDA)


Broth from boiling 150 grams sliced ​​potatoes


in 500ml water for 30 minutes (add water to 500ml)


9 g agar-agar


7 grams of dextrose


1 gram of yeast or yeast extract (optional)




Amaranth Soy Agar


20 grams amaranth flour


20 grams soy flour


9 grams agar-agar


500 ml drinking water or distilled water





Cornmeal-dextrose agar


25 grams yellow cornmeal


3 grams of dextrose


9 grams agar-agar


500 ml drinking water or distilled water





Dog food agar DFA


20 grams of dog food


20 grams of agar-agar


1000 ml distilled water





One thing to note when preparingAgar recipes is that it is definitely a situation where less is more. If too many nutrients added to the agar, it is hypertonic. What this means is there is a higher concentration of particles in the solution, as there are in the dividing cells of our mycelium. Nature takes stock. When mycelium is placed in this hypertonic solution, the equilibrium is trying to assert itself. Since the cell membranes of the mycelium are selectively permeable only tocertain things on the membrane. The water inside the cell will dry out the membrane by osmosis stop outside and cross the cell growth. Therefore, sugar is an energy source for the bacteria, as a preservative in jams and jellies can be used. The jam is hypertonic and inhibits growth.





When preparing agar recipes, you need a container to sterilize the mixture to A q
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uart size jar works perfectly for it. Drill a 3 / 8 "inch hole inthe metal lid and fit inside a Tyvek filter disk. Mix the contents thoroughly into the quart jar. Put the lid loosely on the glass and load in your pressure cooker (optional: you can heat your water to a boil before the quart jar, this will prevent caramelization of the ingredients.). Fill the cooker with water until about 1 "water level is on the side of the glass. Sterilize the jar for 30-40 minutes at 15 psi





AfterSterilization time, let the cooker cool down to release the pressure before opening zero. The cooker should be opened in front of a flow hood or a sterile glove box should be used when pouring agar plates. Each recipe fill up 20 agar plates.





If the glass is cool to touch, but the contents are still liquefied, pour enough to fill MEA or PDA to the bottom of the petri dish. The longer the lid is left open to the air the greater the chance for airContaminants into the culture medium. Let the agar cool to room temperature and solidify. Optional: You can seal the edges with Parafilm to help ward off bacterial contamination.


When inoculating agar plates, again it is important to use a sterile glove box or flow hood to reduce the chances of contamination. Each agar plate with wedges of a colonized agar plate, spores from a mature mushroom or a prepared liquid culture are inoculated.


If youhave purchase our pre-sterilized medium, please follow these instructions to warm up.


Instructions for use of our agar culture medium.


Point your quart jar in a pot with water and fill the pot with water until it covers half of the quart jar.





Leave the lid in place to prevent contamination from entering. Increase the temperature to boiling water to liquefy the medium. Agar will remain liquid up to 96.8 degrees F.


If the mediaLPG can be poured into the waiting agar plates. It is important to pour your agar plates under sterile conditions. We strongly recommend a Flow Hood, but could work a glove compartment. We make no guarantees, if a flow hood is not used. Bacteria is present everywhere, including the air. It only takes a few particles land on your medium to contaminate it. When the agar solidifies again, you can set the margins of the agar plates with parafilm or other sealing device.





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